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The template_WES_standard_curve object provides a template of the data format required by the 'WES' package for standard curve values. These data are only required when calculating the number of gene copies using the calc_n_copies function.

Usage

template_WES_standard_curve

Format

template_WES_standard_curve

A data frame with 3 columns:

target_name

The unique name of the gene target for which the Ct values correspond.

n_copies

The number of gene copies represented in the particular dilution.

ct_value

The Cycle Threshold (Ct) of the qPCR assay.