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Version 1.0.0

CRAN release: 2024-07-12

The ‘WES’ R package v1.0.0 migrated from the ‘es’ R package v1.0.0 on July 03, 2024

Welcome to the first release of the ‘WES’ R package! Version 1.0.0 contains a collection of basic features that aim to make analyzing environmental sampling data easier and more reproducible. The functionality we have included to kick off the package includes:

  • Detailed descriptions of standard data formats for environmental sampling data and standard curve assays. The functionality of the package requires data to follow these formats.
  • Functions for the absolute and relative quantification of qPCR data which include:
    • Calculating the number of gene copies using Ct values and a standard curve assay
    • Calculating the ΔΔCt method for the fold-change in gene expression relative to a reference gene. An efficiency-weighted version is also included.
    • Estimation of the percentile amplification efficiency using a standard curve assay.
  • Functions for downloading covariate data from several open-source APIs and methods for relating these data to environmental sampling observations. These data include:
    • Climate
      • Data from Open-Meteo Historical Weather API
      • cumulative precipitation
      • accumulated thermal units
      • Evaporative Stress Index (ESI)
    • Topology and hydrology
      • Data from Amazon Web Services (AWS) API
      • elevation
      • slope
      • aspect
      • flow accumulation
      • stream networks
      • drainage catchments
    • Local population
      • Data from WorldPop
      • Population counts in drainage catchments
      • Population counts within radius
    • Administrative boundaries
      • Data from geoBoundaries
      • Place names of sampling sites
  • Basic calculation of sample sizes and detection rates